Journal: bioRxiv

Article Title: Methyl gallate Attenuates Post-Stroke Emotional and Cognitive Symptoms by Promoting Hippocampal Neurogenesis via PI3K/GSK3 and AMPK Signaling

doi: 10.1101/2025.07.08.663507

Figure Lengend Snippet: (A) Levels of NSCs marker Sox2 (Red) and immature neural fiber marker DCX (Red). ( B, C) Western blot bands and statistical analysis to show the phosphorylation of GSK3β on Y216 and the total GSK3β protein as well as the phosphorylation of AMPK on Thr172 and total protein level in hippocampal tissue. ( D) DCX levels in cultural neural induction experiments. ( E) Experimental procedure to show the LY294002 or compound (C) treatment in the MET group. ( F, G) Grooming time in the splash test and recognition index in ORT.

Article Snippet: Subsequently, membranes were incubated overnight at 4°C with the following primary antibodies from Cell Signaling Technology (CST): Rabbit anti-pGSK3β (Y216) (1:1000), Rabbit anti-GSK3β (1:1000), Rabbit anti-pAMPKα (Thr172) (1:1000), and Rabbit anti-AMPKα (1:1000).

Techniques: Marker, Western Blot, Phospho-proteomics

Journal: Physiological Reports

Article Title: Subtherapeutic lithium supplementation causes physiological eccentric cardiac hypertrophy in young‐adult wild‐type male mice

doi: 10.14814/phy2.70299

Figure Lengend Snippet: GSK3, SERCA, and PLN content are unchanged with 12 weeks LiCl treatment. Representative Western blots of phosphorylated (Ser9) GSK3β (pGSK3β) and total GSK3β (tGSK3β) (a). Optical density analysis of pGSK3β (b), tGSK3β (c), and their ratio (d). Representative blots of SERCA2 and PLN (e). Optical density analysis of SERCA2 (f), PLN (g), and their ratio (h). For (b–d) and (f–h), a two‐tailed Student's t ‐test was used with exact p values shown for statistically significant ( p < 0.05) or trending ( p < 0.10) comparisons. All values represented as mean ± SD, n = 5–6 per group.

Article Snippet: SERCA2 (MA3‐919; ThermoFisher Scientific; Waltham, MA, USA), PLN (MA3‐922; ThermoFisher Scientific), and total GSK3β (9315; Cell Signaling Technology; Beverly, MA, USA) antibodies were incubated with 5% milk, while pGSK3β (Ser9; 9336; Cell Signaling Technology) antibody was incubated with 3% BSA.

Techniques: Western Blot, Two Tailed Test